Identification of a novel biomarker for Herceptin resistance in HER2 postivie breast cancer cells
Incheol Shin
Incheol Shin
Department of Life Science
Hanyang University
Seoul, South Korea
Abstract:
In an attempt to find novel protein biomarker(s) associated with Herceptin resistance in breast cancer, we have generated herceptin-resistant (HR) HER2-positive breast cancer cells from mouse xenograft model. The protein expression profiles of parental and HR cells were analyzed by differential proteomics using LC/MS-MS approaches. We have identified 688 differentially expressed proteins in parental and HR breast cancer cell lysates and 308 proteins in secretome. Some of the best candidate proteins were knocked-down in HR cells and their effect on cell proliferation, intracellular signaling, herceptin-resistance was investigated. The possible roles of the biomarker proteins in maintaining resistance to herceptin and their potential as a novel therapeutic candidates for herceptin-resistant breast cancer will be discussed.
